<i>ism1</i> knockdown decreases neutrophils and macrophages in 48 hpf zebrafish.

<p><i>mpx</i>:GFP single-cell-stage embryos were injected with 7 ng <i>ism1</i> MO (MO; squares), or 7 ng <i>ism1</i> MO and 17.88 ng of <i>ism1</i> mRNA (Rescue; triangles); uninjected embryos served as controls (circles). (<b>A</b>) Developing 48 hpf zebrafish were visualized, and individual <i>mpx</i>:GFP⁺ cells were enumerated; representative fish shown for reference. (<b>B</b>) Each data point represents total amount of <i>mpx</i>:GFP⁺ cells present in one zebrafish. (<b>C</b>) qRT-PCR was performed comparing transcript levels of <i>csf3r</i> (left) and <i>csf1r</i> (right) in <i>ism1</i> MO injected (MO) and uninjected controls (control) at 48 hpf. Bars represent the mean, and error bars represent standard deviation. A two-tailed unpaired Student’s T test was performed to determine statistical significance. * denotes p<0.05, ** denotes p<0.0001, N.S indicates no significance.