Detection of morphological haploinsufficiency.

<p>(A) Graphical representation of heterozygous essential gene-deletion mutants exhibiting haploinsufficiency phenotypes (<a href="http://www.plosbiology.org/article/info:doi/10.1371/journal.pbio.2005130#pbio.2005130.s024" target="_blank">S1</a> and <a href="http://www.plosbiology.org/article/info:doi/10.1371/journal.pbio.2005130#pbio.2005130.s025" target="_blank">S2</a> Data). The size of the colored area shows the relative ratio of the corresponding heterozygotes. (B) Percentage of heterozygotes exhibiting haploinsufficiency in at least 1 morphological trait (FDR = 0.01; <i>P</i> < 7.64 × 10⁻⁵) using the morphological traits examined. Red and gray lines indicate essential and nonessential genes, respectively. The orange line indicates the percentage of FPs in 114 wild-type replicates. Black solid line indicates percentage of samples detected by chance as estimated using parametric bootstrap resampling. Black dashed line indicates 50%. The morphological traits were ordered on the x-axis by randomizing the 501 traits (3,000 iterations). FDR, false discovery rate; FP, false positive.