Proliferation and cell death during embryonic kidney development.

<p>A. Proliferating cells were detected with phosphohistone 3 antibody in kidneys at E14.5. Proliferation rates over comparable areas are similar between normal and mutant kidneys. B. TUNEL analysis of kidney at E14.5. The incidence of TUNEL-+ cells at this stage over comparable areas is similar between normal and mutant kidneys. C. immunostaining for anti-phosphohistone 3 in proliferating cells in kidneys at E11.5. The dotted line encircles comparable portions of metanephroi. D. TUNEL analysis of kidney at E10.5 (upper) and E11.5 (lower). The kidney is stained with a rabbit anti-Pax2 antibody to demarcate the MM/UB area. The epithelial structure is the nephric duct (ND), and the adjacent Pax2+ tissue delineates the MM region. The UB is also marked with Hoxb7/myr-venus (A, B, D). TUNEL+ cells are numerous in mutant MMs. nor—normal, mu—mutant.