Optimization of Flow Rate and Column Temperature for Simultaneous Determination of Six Food Additives by UFLC

The column temperature and flow rate are two important variables to determine the speed of separation of compounds in the mixture. The study was conducted to determine the optimum column temperature and flow rate on the separation of food additives mixture containing saccharin, cyclamate, benzoate, sorbate, tartrazine and yellow sunset. Research using high-performance liquid chromatography, reversed phase with the instrument: UFLC 1290 DAD, C18 column 100 mm x 4.6 mm x 3.5 μm. The optimization parameters include the capacity factor, theoretical plate number, resolution, selectivity and tailing factor. The results showed that the optimum column temperature and flow rates were 30^oC and 1.0 ml/min respectively with wavelengths of 200, 220 and 450 nm at the composition of phosphate buffer pH 4.5 - methanol was 75: 25