AIH treatment for 7 days increased BDNF protein levels in multiple spinal segments.

<p>Representative photomicrographs of the ventral grey matter in C6-7 (<b>A</b>) or L4-5 (<b>B</b>) spinal segments sections processed for BDNF immunofluorescence from 1 day (Day-1) or 7 day (Day-7) in normoxia-treated versus AIH-treated spinal injured rats. Arrows indicate representative neurons. Scale bar = 50 μm. Histograms summarize the mean immunofluorescence signal intensity detected ± SEM as measured in gray values over individual neurons within the ventral horn from normoxia- and AIH-treated spinal injured rats [n = 3 rats per experimental group analysed; 60–65 neurons analysed/time point/experimental condition at C6-7 (<b>A</b>); 80–85 neurons analysed/time point/experimental condition at L4-5 (<b>B</b>)]. Asterisks indicate significant differences between experimental groups; * p < 0.05.