AQP3 localizes to the PVM and auphen treatment delays <i>P</i>. <i>falciparum</i> development in erythrocytes.

<p>(A) Fluorescent microscopy of erythrocytes infected with <i>P</i>. <i>falciparum</i> 3D7. Cells were stained with <i>Hs</i>AQP3 (red) and Hoechst (blue). AQP3 localizes to the PV of infected cells. (B) Schematic for auphen treatment of <i>P</i>. <i>falciparum</i> infected erythrocytes. Cells were treated with 2 μM auphen at the early schizont stage for 40 hours. (C) Parasitemia in auphen-treated cultures decreased 75 ± 12% compared to DMSO treated <i>P</i>. <i>falciparum-</i>infected erythrocytes (*<i>p</i> = 0.0257, unpaired Student’s <i>t-</i>test; n = 3 independent experiments). (D) After 40 hours of auphen treatment parasites were scored as ring, trophozoite, or schizont parasites. Auphen-treated <i>P</i>. <i>falciparum</i> exhibited a greater population of ring stage parasites when compared to the DMSO-treated control (n = 1 independent experiment).