Simulating MT array dissolution from interphase to prophase (NEBD).

<p>(A) Parameter Set A was used to build an interphase array and then dynamic instability was switched to parameters measured at around NEBD (<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0197538#pone.0197538.t002" target="_blank">Table 2</a>). The parameters measured at NEBD promote rapid loss of MTs at rates consistent with the rapid process estimated LLCPK1 cells. Increasing nucleation rate 5-fold, as measured at around NEBD in these cells [<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0197538#pone.0197538.ref022" target="_blank">22</a>] did not make a significant contribution to the shape of the array. (B) Simulating the switch to prophase dynamics, but keeping either catastrophe or rescue at their interphase rates showed that keeping rescue at its interphase rate was sufficient to maintain the interphase array and prevent array dissolution, indicating that the reduced rescue at prophase is the critical parameter allowing disassembly of the MT array as cells prepare to enter mitosis.