Rosmarinic acid derivatives also inhibit ε-Pol binding.

<p>(A) Western blot analysis for Pol pulled-down by ε-biotin in the presence of the indicated compounds. Arrows: 3×FLAG-Pol. (B) ssDNA-biotin was pulled-down in the presence of the indicated compounds, and detected by EtBr staining. Arrow: 71-base ssDNA. (C) dsRNA-RIG-I EMSA in the presence of the indicated compounds (100 μM). *: monomeric dsRNA, **: the dsRNA-RIG-I complex. (D) Hep38.7-Tet cells were treated with 10 μM rosmarinic acid, compounds 12c, 13c, 2, and 21a, 100 nM lamivudine, or 400 ng/ml tetracycline. Extracellular HBV DNA and cell viability were measured as in (<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0197664#pone.0197664.g003" target="_blank">Fig 3A and 3B</a>). Data are from one representative of at least two independent experiments; the means and S.D. of triplicate experiments are shown (* <i>p</i> < 0.05, ** <i>p</i> < 0.01, *** <i>p</i> < 0.001). (E) A Western blot analysis for Pol pulled-down by ε-biotin in the presence of the indicated compounds. Arrows: 3×FLAG-Pol. Spliced images are indicated with a dividing line.