Multiple sequence alignment of the investigated <i>E</i>. <i>huxleyi</i> CBM1 domains and selected related sequences.

<p>In this MSA, the six <i>E</i>. <i>huxleyi</i> CBM1 domains are shown together with predicted non-fungal CBM1 domains, a selection of biochemically characterized CBMs from the CAZy database: CBM5 and fungal CBM1. The consensus sequence is visualized underneath the MSA. Residues are colored according to Zappo color scheme, with conserved residues colored more intensely. The three key aromatic residues involved in cellulose binding (position 7, 35, 38) are indicated with yellow triangles above the sequences. Alignment made with MUSCLE and formatted with Jalview 2 [<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0197875#pone.0197875.ref032" target="_blank">32</a>,<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0197875#pone.0197875.ref033" target="_blank">33</a>].