Expression of genes associated with proliferation and stem like function.

<p>A) Overlap between genes with increased expression (log2 fold change > +1.0, p adjusted < 0.0001) in tumors compared to wild type for each of the four tumor groups shown. B) Enrichment analysis for putative transcription factor binding and GO biological process terms in genes with increased expression in double mutant tumors or in all four groups. C) Overlap between genes with lower expression (log2 fold change < -1.0, p adjusted < 0.0001) in tumors compared to wild type for each of the four tumor groups shown. D) Heat-map showing relative changes in expression for a selection of genes with links to stem cell-like function in cancer. E) A selection of the gene expression changes from D were validated by qRT-PCR in Pten and Pten;Tgfbr2 tumors (N = 4 each) shown as mean + sd. F) GSEA analysis of the CIN70 dataset that is associated with more aggressive tumors. G) qRT-PCR validation in Pten and Pten;Tgfbr2 tumors of a selection of genes from the CIN70 set and from the other GSEA shown in <a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1007409#pgen.1007409.s001" target="_blank">S1 Fig</a>. * p < 0.05 by student’s T test.