Western immunoblots shows dose dependent response of fulvestrant on relative abundance of proteins (IRS-1, IGF-1R, PDZK1 and ER-α) in MCF-7 cell lysates.

<p>(A) Lane 1 shows the control. Lane 2, 3 represents the protein expression profile of breast cancer cell line and following treatment with fulvestrant (0.001–0.01) for 48hrs. GAPDH was assessed as a loading control. Cells were incubated with antibodies and fixed against IRS-1, IGF-1R, PDZK1 and ER-α. Protein bands were measured and normalized to GAPDH or total individual protein expression. (B) Relative expressions of proteins compared with loading control. Asterisks shows the statistical significance of proteins cultured in serum containing medium in MCF-7 cells (unpaired <i>t</i> test; IRS-1 ***<i>p</i> < 0.0002, IGF-1R **<i>p</i> < 0.0011, PDZK1 ***<i>p</i> < 0.0004 and ER-α ***<i>p</i> < 0.0008). (C) Relative abundance of dose dependent proteins was expressed as %age of maximum value measured as means ± SEM from three replicate for each blot experiment. Asterisks indicate the statistical significance of proteins cultured in serum containing treated medium in MCF-7 cells (One-way ANOVA; *<i>p</i> < 0.01).