Phylogenetic analysis of the partial COPA sequence obtained from goldfish.

<p><b>(A)</b> Phylogenetic tree showing the evolutionary relationships of the obtained nucleotide sequence of goldfish <i>copa</i> with those of other species. Tree was inferred by the neighbor-joining method using the online tool <a href="http://www.phylogeny.fr" target="_blank">www.phylogeny.fr</a>. The scale bar indicates the average number of substitutions per position (a relative measure of evolutionary distance). The names of the species used for the alignment are provided in the figure. GenBank accession numbers of the sequences used are as follows: <i>Bombyx mori</i>, NM_001172721.1; <i>Bos taurus</i>, NM_001105645.1; <i>Carassius auratus</i>, JQ929912.1; <i>Cavia porcellus</i>, XM_003466569.3; <i>Ciona intestinalis</i>, XM_002131228.4; <i>Cricetulus griseus</i>, XM_007629307.2; <i>Danio rerio</i>, NM_001001941.2; <i>Equus caballus</i>, XM_023640888.1; <i>Gallus gallus</i>, NM_001031405.2; <i>Homo sapiens</i>, NM_001098398.1; <i>Loxodonta africana</i>, XM_023554162.1; <i>Macaca mulatta</i>, XM_015113467.1; <i>Meleagris gallopavo</i>, XM_003213951.3; <i>Monodelphis domestica</i>, XM_016430274.1; <i>Mus musculus</i>, NM_009938.4; <i>Nomascus leucogenys</i>, XM_012510889.1; <i>Pan troglodytes</i>, XM_001171563.4; <i>Rattus norvegicus</i>, NM_001134540.1; <i>Saccoglossus kowalevskii</i>, XM_002731243.2; <i>Salmo salar</i>, XM_014140665.1; <i>Sus scrofa</i>, XM_001928697.6; <i>Thalassiosira pseudonana</i>, XM_002291058.1; <i>Xenopus laevis</i>, NM_001093019.2; <i>Xenopus tropicalis</i>, NM_001127994.1. <b>(B)</b> Alignment of the first 61 aa of COPA from an algal species, invertebrate species, teleost fishes, amphibians, avians, and mammals. The species names are provided on the left-hand side of the alignment and the number of aa is present on the right-hand side of the alignment. The coloured aa highlights the differences in conservation between species. The first 25 aa (which correspond to the xenin region) are boxed.