Flow cytometry and microscopic observation of cardiac cell sheets.

<p>(A) A histogram of fluorescently labeled human iPSC-derived cells after twice puromycin treatments, analyzed by flow cytometry. The purity of cardiomyocytes (cardiac TnT-positive cells) was confirmed. (B) A phase-contrast microscopic image of a cardiac cell sheet cultured on a temperature-responsive dish at 7 days after cell seeding. (C) A phase-contrast microscopic image of a cardiac cell sheet transferred on a fibrin gel sheet at 3 days after cell sheet transfer. (D) A microscopic image of H&E stained paraffin section of a cardiac cell sheet on a fibrin gel sheet after cell sheet transfer.