Localization of FtsZ and Pbp2b during filament formation.

<p><i>L</i>. <i>lactis</i> cells expressing FtsZ-Ve (NZ3900 [pGIBLD031]) (A) or Ve-PBP2b (NZ3900 [pGIBLD041]) (B) were grown in the presence of methicillin 1 μg ml^-1 to induce filament formation and visualized by phase contrast (PC) and fluorescence microscopy to examine the subcellular localization of the corresponding proteins at different stages of filament elongation. Schematic diagrams summarizing the data are shown below the microscopy pictures. The localization pattern of FtsZ-Ve and Ve-PBP2b are shown in green and yellow, respectively. Scale bars, 2 μm.