Biomarker-Based Metabolic Labeling for Redirected and Enhanced Immune Response

Installation of an antibody-recruiting moiety on the surface of disease-relevant cells can lead to the selective destruction of targets by the immune system. Such an approach can be an alternative strategy to traditional chemotherapeutics in cancer therapy and possibly other diseases. Herein we describe the development of a new strategy to selectively label targets with an antibody-recruiting moiety through its covalent and stable installation, complementing existing methods of employing reversible binding. This is achieved through selective delivery of 1,3,4-<i>O</i>-acetyl-<i>N</i>-azidoacetylmannosamine (Ac₃ManNAz) to folate receptor-overexpressing cells using an Ac₃ManNAz-folate conjugate <i>via</i> a cleavable linker. As such, Ac₃ManNAz is converted to cell surface glycan bearing an azido group, which serves as an anchor to introduce l-rhamnose (Rha), a hapten, <i>via</i> a click reaction with aza-dibenzocyclooctyne (DBCO)-Rha. We tested this method in several cell lines including KB, HEK-293, and MCF7 and were able to demonstrate the following: 1) Rha can be selectively installed to the folate receptor overexpressing cell surface and 2) the Rha installed on the target surface can recruit anti-rhamnose (anti-Rha) antibodies, leading to the destruction of target cells <i>via</i> complement-dependent cytotoxicity (CDC) and antibody-dependent cellular phagocytosis (ADCP)