Movie 4 related to fig2c: Basal extrusion of GFP-CEP131 expressing cell from MDCK 2D monolayer. from Structural centrosome aberrations sensitize polarized epithelia to basal cell extrusion

Movie shows brightfield signal (upper movie) and corresponding fluorescence signals (lower movie) representing mCardinal-ZO-1 (red) and GFP-CEP131 (green) (lower movie). MDCK were cells cultured in 2D monolayer, induced to express GFP-CEP131 for 48 hours and treated with etoposide to stimulate cell extrusion. Images were acquired every 20 minutes and movie displays 1 frame per second. Scale bars represent 10 microns