Histone H3 and H4 core modifiable alleles that suppress <i>cdc6-4</i> temperature sensitivity define a known Sir3-nucleosome interface.

<p><b>A.</b> List of histone H3 and H4 substitutions within the mutant library that were screened for their ability to affect the growth of <i>cdc6-4</i> mutant cells (see also <b><a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1007418#pgen.1007418.s009" target="_blank">S9 Fig</a></b>). <b>B.</b> 10-fold serial dilutions of M1345 (<i>cdc6-4</i>) with either wild type H3, H4 or selected histone variants that were from the original library (<b>Fig 6A</b>) or were generated by site-directed mutagenesis (see text), were spotted onto YPD and grown at the indicated temperatures. Asterisks (*) indicate the histone variants that suppressed <i>cdc6-4</i> temperature-sensitive growth. H4K59A and H4K59R are shown as examples of histone mutants that did not suppress. <b>C.</b> Nucleosome structure (PDB-1ID3) with one H3(dark blue)-H4(light blue) dimer and the H3K79, H3T80, H4E74 and H4K79 residues highlighted in red.