Western blot analysis of proteinase K-treated homogenates from brainstems of goats intracerebrally inoculated with C-BSE.
- Elena Vallino Costassa (2610193)
- Antonio D’Angelo (4605814)
- Maria Mazza (332097)
- Daniela Meloni (137109)
- Elisa Baioni (5278625)
- Cristiana Maurella (5278622)
- Silvia Colussi (4916896)
- Nicola Martinelli (42974)
- Monica Lo Faro (5278628)
- Elena Berrone (5278619)
- Alessandra Favole (5278616)
- Paola Crociara (410239)
- Silvia Grifoni (5278631)
- Marina Gallo (5278634)
- Guerino Lombardi (47142)
- Barbara Iulini (47153)
- Cristina Casalone (47143)
- Cristiano Corona (47148)
Publication date
May 2018
DOIAbstract
<p>I: C- BSE (16819/06) isolate used for <i>inoculum</i>; lanes 1–5: inoculated goats; gs: goat with natural classical scrapie. MW: molecular size markers. Membranes were probed with three different monoclonal antibodies: P4 (A), 6H4 (B), and SAF84 (C). Panel D shows the proteinase K-treated homogenates treated with enzymatic deglycosylation (PNGase) and detected with mAb SAF84.</p
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