Albumin led to the reduction of intracellular superoxide anion level induced by hydrogen peroxide in mouse mesangial cells.

<p>(A) Representative image of intracellular superoxide anion detected by dihydroethidium (DHE) staining in SV40 MES-13 cells after 6-h exposure to hydrogen peroxide (500 μM) with or without bovine serum albumin (BSA, 3.0 g/dL). As a control (CTR), we used the cells that were not exposed to hydrogen peroxide. (B, C) The DHE fluorescent intensity per cell of SV40 MES-13 cells that were exposed to hydrogen peroxide with or without BSA or with γ-globulin. MES-13 cells were exposed to 500 μM hydrogen peroxide for 6 h with or without BSA (0.75, 1.5, 3.0, and 4.5 g/dL) or with 3.0 g/dL γ-globulin, and the DHE fluorescent intensity per cell was determined by the fluorescent plate reader. As a CTR, we used the cells that were not exposed to hydrogen peroxide. Data are presented as the mean ± standard error of three independent experiments, each of which was repeated at least three times with similar results obtained. * <i>P</i> <0.05 vs. CTR, † <i>P</i> <0.05 vs. H₂O₂ 500 μM, ‡ <i>P</i> <0.05 vs. H₂O₂ 500 μM + γ-globulin.