Control of intramacrophage LVS growth depends on T cells but not B cells.

<p>BMMΦ from Fischer 344 rats were infected with LVS and co-cultured with total, CD45R⁺ (B lymphocytes) or CD45R^- splenocytes (non-B lymphocytes) obtained from naïve or vaccinated Fischer 344 rats, as indicated. Splenocytes were analyzed by flow cytometry to evaluate purity of B and T cells, as indicated. After three days of co-culture, BMMΦ were lysed to evaluate the recovery of intracellular bacteria (Panel A). Supernatants were collected for analyses of IFN-γ by ELISA (Panel B) and NO by Griess reaction (Panel C), and their concentrations were calculated using standard curves as references. Values shown are the mean concentration ± standard deviation of triplicate wells. Brackets indicate significant differences between the indicated groups (<i>P</i> < 0.05). Data shown are from one representative experiments of two of similar design and outcome.