Combination therapy with GDC0941 and AS-605 demonstrates <i>in vitro</i> efficacy.

<p><b>A.</b> Table of IC50 values for GDC-0941 and AS-605 showing effect on different PI3K isoforms. Source: Selleckchem.com <b>B.</b> Cells were seeded in the presence of each inhibitor or the combination. Bar graphs show the effect of combining GDC0941 and AS-605 inhibitors on growth of 1156 and 1713 T-ALL cell lines. Cell counts of the DMSO-treated control were set at 100% and the effect of each of treatment was calculated as a proportion of that. <b>C.</b> Histograms showing baseline phospho-Akt (S473) levels in human T-ALL cell lines (red line). Grey-filled histograms depict unstained controls. <b>D.</b> Bar graphs showing the effect of combining GDC0941 and AS-605 inhibitors on growth of human T-ALL cell lines. As in (B) cell counts of the DMSO treated control were set at 100% and the effect of each of treatment was calculated as a proportion of that.