Phylogenetic tree of HBV isolated from Amerindian Warao population.

<p>A) Phylogenetic analysis of HBV S region (658 bp) and B) of two complete genome HBV isolated of Amerindian Warao population. Genetic distance was estimated by Kimura two parameters correction, MEGA version 7.0.26. Phylogenetic tree was constructed with the Neighbour-joining method. Numbers at each node correspond to bootstrap values (>50%) obtained with 1000 replicates. The prototype strains are designated by their GenBank accession number, and country or ethnic group, except the Warao isolates of this study (bold). VNZ: Venezuela. *These Warao isolates were obtained between the years 1991–1997 and analyzed in a previous study [<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0197662#pone.0197662.ref034" target="_blank">34</a>]. The sequences of occult HBV infection are underlined. Six isolates for which only a 330 pb fragment could be amplified are not included in the tree.