Additional file 4: of Systemic overexpression of SQSTM1/p62 accelerates disease onset in a SOD1H46R-expressing ALS mouse model

Figure S4. Immunoblot-images used for the quantitative analysis in Figs. 6a and Fig. 7. The immunoblots of (a) soluble SOD1, (b) insoluble SOD1 monomer (Mono), (c) insoluble high-molecular weight (HMW) SOD1, (d) soluble SQSTM1, (e) insoluble SQSTM1, (f) soluble poly-ubiquitinated proteins, (g) insoluble poly-ubiquitinated proteins, (h) soluble GAPDH, and (i) insoluble actin were analyzed for the quantitative analysis (Figs. 6a and 7). The spinal cord from wild-type (WT), SQSTM1 (SQSTM1), SOD1 H46R (H46R), and SQSTM1;SOD1 H46R (SQSTM1;H46R) mice at 16 weeks of age (wk), 22 wk., and end-stage (H46R and SQSTM1;H46R) or 28 wk. (WT and SQSTM1) were used. C (control sample) used as internal control indicates soluble and insoluble fractions from 22 week-old SQSTM1;SOD1 H46R mouse. Asterisk represents the measured area of poly-ubiquitinated proteins. (PDF 1973 kb