Additional file 1: of Light sheet theta microscopy for rapid high-resolution imaging of large biological samples

Figure S1. LSTM microscopy implementation. (a) Image of the physical LSTM setup. (b) 3D model of LSTM and the sample mounting system. The 3D-printed sample chamber is designed to accommodate large biological samples of virtually any dimensions, while still allowing the objectives to be immersed in the immersion oil. Two transparent glass windows, located on the lateral sides, provide visual view of the sample for ease of positioning. An additional window is realized at the bottom part of the chamber to allow the illumination light to pass through. An additional adapter was designed to allow mounting a prism mirror at about approximately 10° from the normal surface to facilitate the optical alignment of the system. (PDF 1623 kb