Additional file 1: of Systemic overexpression of SQSTM1/p62 accelerates disease onset in a SOD1H46R-expressing ALS mouse model

Figure S1. Schema of the transgene construct for SQSTM1-tg mouse and the distribution of SQSTM1 in tissues. a Schematic diagram of the transgene cassette encoding the C-terminally hemagglutinin (HA)-tagged human SQSTM1 cDNA. Transgene construct consists of cytomegalovirus enhancer (E), chicken β-actin promoter (Pro), rabbit β-globin splice acceptor (S), full length human SQSTM1 cDNA (SQSTM1), hemagglutinin tag (HA), and rabbit β-globin poly A (Poly A). b Expression and distribution of SQSTM1 in the liver, skeletal muscle, olfactory bulb, cerebral cortex, hippocampus, cerebellum, and spinal cord in wild-type (WT) and SQSTM1-tg (SQSTM1) mice. Expression of mouse endogenous SQSTM1 and human SQSTM1 (Endogenous + SQSTM1-HA) was simultaneously detected by western blotting using anti-SQSTM1 antibody. Values shown under each lane were the signal intensities in an arbitrary unit relative to that of the liver in WT mouse. Human SQSTM1 (hSQSTM1-HA) was specifically detected by anti-HA antibody. GAPDH was used as a loading control. (PDF 795 kb