<i>In vitro</i> characterization of PDGFRβ-selected cells isolated from lungs of Pdgfrb-Cre+;<i>Itga8</i>^{<i>flox/-</i>} (Cre+) and WT or <i>Itga8</i>^{<i>flox/flox</i>} (Cre-) mice.

<p>(A) Western blot showing the absence of ITGA8 expression in Cre+ cells compared to Cre- cells. (B) Adhesion assay showing decreased binding to the substrates nephronectin (NPNT) and fibronectin (FN) by Cre+ cells. (C-G) PDGFRβ-selected cells were treated with TGFβ (10 ng/ml) or vehicle. mRNA expression is presented as fold change relative to Cre- in the untreated group (vehicle). Genes include: <i>Itga8</i> (C), <i>Col1a1</i> (D), <i>Acta2</i> (E), <i>Ctgf</i> (F), and <i>Fn1</i> (G). (*<i>p</i><0.05, mean±SEM, n = 4).