Effect of inhibiting viral DNA replication on co-localization of BMRF1 and BGLF4 during EBV lytic replication.

<p>EBV B95-8 bacmid 2089 infected 293 cells were transfected with Zta expression vector (+Z) to induce EBV lytic replication and mock-treated or treated with PAA to block viral replication after replicative complex formation, at 24 hr or 48 hr post-induction. Cells were then stained for BGLF4 (Red) and BMRF1 (Green) at the indicated times post induction. All PAA treated cells were fixed and stained at 96 hr post-induction. BMRF1, the polymerase-associated processivity factor was used as a marker for viral replication foci.