Analysis of the endothelial cell gene expression profile following treatment with EVs isolated from <i>Mtb</i>-infected and uninfected macrophages.

<p>Total RNA was sequenced for two independent biology replicates. <b>(A)</b> Venn diagram of the genes that showed a minimum two fold up- or down-regulation in endothelial cells following treatment with EVs from <i>Mtb</i>-infected (RvEV) or uninfected (UnEV) macrophages compared to each other and to untreated cells. <b>(B)</b> Hierarchical cluster analysis of differentially expressed genes in endothelial cells treated with EVs isolated from <i>Mtb</i>-infected or uninfected macrophages. The analysis was conducted with a minimal 2-fold change compared with resting endothelial cells.