Analysis of mating-type switching directionality of Set1C mutants.

<p>(A, B) Spore formation of mutants in each Set1C subunit was assayed by exposure of colonies to iodine vapor. <i>h</i>^<i>90</i> strains are shown in (A) and <i>h</i>^<i>09</i> stains are shown in (B). (C, D, E) Quantification of <i>mat1</i> content estimated by multiplex PCR. The relative P band intensity in each lane (P/(P+M)) was calculated from the data shown in <a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1007424#pgen.1007424.s005" target="_blank">S5 Fig</a> for the <i>h</i>^<i>90</i> strains shown in (C); from <a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1007424#pgen.1007424.s005" target="_blank">S5 Fig</a> for the <i>h</i>^<i>09</i> strains shown in (D) and from <a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1007424#pgen.1007424.s005" target="_blank">S5 Fig</a> for the strains with mutated <i>SRE</i> elements shown in (E-H). (E) 2×<i>SRE2</i> strains (<i>mat2-P-SRE2 mat3-M-SRE2</i>) were derived from the <i>set1</i>⁺ strain TP126; (F) 2×<i>SRE3</i> strains (<i>mat2-P-SRE3 mat3-M-SRE3</i>) from TP303; (G) <i>SRE3Δ</i> from TP75; and (H) <i>SRE2Δ</i> from TP8. Four <i>set1</i>Δ isolates are shown in each case. (C-H) Data are represented as mean ± SD. Two-tailed paired Student’s t test was used to compare the mean of each sample to cntl, *p < 0.01; **p < 0.005; n.s., not significant.