Impact of Cafeteria Diet on the Composition of Fatty Acids in Zebrafish (Danio rerio) Fillets

<div><p>The cafeteria diet is widely used as a model for inducing obesity in animals, and the zebrafish (Danio rerio) has emerged as a promising model for this purpose. In this context, this study evaluated the effect of a cafeteria diet on the composition of fatty acids in zebrafish fillets. Zebrafish grown in tanks for a period of 60 days were treated with standard (ST) and cafeteria (CAF) diets. The CAF diet presented higher total lipid and energy values than ST diet, due to the addition of peanut, chocolate and biscuit in its preparation. Moreover, CAF diet showed the highest concentrations of palmitic (19.44 ± 0.55 mg g-1), oleic (102.58 ± 2.85 mg g-1) and linoleic (44.47 ± 1.32 mg g-1) acids. A significant increase in oleic and linoleic acid concentrations (39.77 ± 2.69 and 14.31 ± 0.86 mg g-1, respectively) and in the omega-6/omega-3 ratio (11.52 ± 0.81) was observed in the zebrafish fillets between 0 and 60 days for CAF diet, indicating incorporation of these fatty acids.</p>