Additional file 4: of The Trem2 R47H variant confers loss-of-function-like phenotypes in Alzheimer’s disease

Figure S3. (A) Expression of amyloid precursor protein (App) and related genes were assessed in cortical lysates from APPPS1–21;Trem2+/+ (n = 13), APPPS1–21;Trem2+/− (n = 13), and APPPS1–21;Trem2 +/R47H (n = 8) mice. Data are presented as fold change normalized gene expression. (B) ELISAs for Aβ1–40 and Aβ1–42 and (C) ratio of Aβ1–42/Aβ1–40 were performed on DEA (soluble) and FA (insoluble) fractions from cortex and hippocampus from APPPS1–21;Trem2 +/+ (n = 17), APPPS1–21;Trem2 +/− (n = 14), and APPPS1–21;Trem2 +/R47H (n = 10) mice. Data are presented as fold change normalized protein expression. *p < 0.05, **p < 0.01. (TIF 9131 kb