KAN0439834 and anti-ROR1 mAb inhibited ROR1 phosphorylation in pancreatic carcinoma cells (PaCa-2).

<p>Total ROR1 (ROR1) and phosphorylated ROR1 (pROR1) after 2 h incubation with KAN439834 (A) and (B) anti-ROR1 mAb. Intensity values of pROR1 relative to total ROR1 are shown. Effects on phosphorylation of RTKs (Phospho-RTK array) in PaCa-2 cells by KAN0439834. (C) PaCa-2 cells were treated with 500 nM of KAN0439834 for 2 h ((■) or untreated (UT) for 2 h (□)). EGFR, ROR1 and IGF-1R were detected in this specific array. Inhibition of ROR1 phosphorylation could only be noted after exposure to KAN0439834. (D) Relative intensity values of pEGFR, pIGF-1R and pROR1 to total EGFR, IGF-1R and ROR1.