Drought and salt stress tolerance of <i>ZmPIP1;1</i> overexpression transgenic lines.

<p>Five-day-old WT and <i>ZmPIP1;1</i>-Oe transgenic <i>Arabidopsis</i> seedlings were treated with 150 mM Manntiol or 100 mM NaCl in MS medium for 7 days. (A) Photographs of WT and <i>ZmPIP1;1</i>-Oe transgenic <i>Arabidopsis</i> seedlings with 150 mM Manntiol or 100 mM NaCl in MS medium for 7 days. Bar = 1 cm. (B) Primary root length in WT and <i>ZmPIP1;1</i>-Oe with 150 mM Manntiol or 100 mM NaCl in MS medium for 7 days. All data are means of 15 biological replicates with error bars indicating SD, *P<0.01. (C) qRT-PCR analysis of three representative <i>ZmPIP1;1</i> overexpression transgenic lines. RNA was extracted from the leaves of fourteen-day-old seedlings. All data are means of three biological replicates with error bars indicating SD. Expression of <i>AtACT2</i> was used as the internal control.