Antibody response against protease and reverse transcriptase assayed by ELISA.

<p>Antibody response in mice receiving PR and RT DNA by intradermal (skin) and intramuscular (muscle) delivery. Titer of IgG against PR (A), IgG, IgG1 and IgG2a against RT (B), and the ratio of anti-RT IgG2a/IgG1 (C). Mice (n = 5 per group) were immunized as described in the legend to <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0197902#pone.0197902.g001" target="_blank">Fig 1</a> and their responses were assessed on experimental end-point at day 21 by indirect ELISA on plates coated with recombinant PR (A), or RT (B, C) of HIV-1 HXB2 (NIH AIDS Reagent Program, Germantown, MD). Titer values represent average per group with SE. Statistical comparison was done using Mann Whitney U-test; *<i>p</i> < 0.05; **<i>p</i> < 0.01; ***<i>p</i> < 0.001; ****<i>p</i> < 0.0001.