Inhibition of cell migration in breast cancer cell lines by wound healing assay after <i>H</i>. <i>salsugineum</i> methanolic extract treatment.

<p>Cells were scratched and treated with 350 μg/ml <i>H</i>. <i>salsugineum</i> methanolic extract for 48h. (A) Representative images were indicating wounded areas before and after treating of the cells. Closure rates were analyzed with ImageJ software. Bar graphs shows the mean of closure rates in (B) MCF-7 and (C) MDA-MB-231. The mean values and the ± standard deviation were obtained from three independent experiments. P<0.05 is considered as statistically significant. *P<0.01 compared to control.