Dense and light core medium size vesicles.

<p>(A) Diameter distribution for dense and light core medium size vesicles. (B) Tomographic slices and corresponding radially averaged density traces (lower values indicate higher density) for a dense core with halo (top), a dense core without halo (middle) and a light core vesicle (bottom). Arrows show the light halo and the corresponding peak, while ‘lum’, ‘m’ and ‘cyto’ denote the position of vesicle lumen, membrane and the surrounding cytoplasm, respectively. Scale bars for traces are shown above them. (C) Distance to the nearest microtubule for all dense core, 80 nm vesicles. (D) Tomographic slices showing dense core vesicle protrusions (long black arrows, short black arrowheads), connectors to synaptic vesicles (white arrowheads) and connectors to other medium size vesicles (white arrow). All tomographic slices are 3.2 nm thick. Scale bars for all tomographic slices 100 nm.