MIF-expressing tumor cells show decreased markers of immunogenic cell death under serum-free conditions.

<p><b>A-C,</b> WT or MIF KD 4T1 cells were grown in 10% serum-containing media overnight, and then media was replaced with fresh 10% serum or serum-free media. After 24 hours, media was sampled and tested for extracellular ATP. After 48 hours, cells were harvested and stained by flow cytometry for cell surface expression of calreticulin or HSP70. <b>D-F,</b> MIF KD 4T1 cells reconstituted (recon) with WT MIF, or P2G MIF, or with an empty vector as a control were grown in 10% serum-containing media overnight, and then switched to serum-free media. After 48 hours, media was sampled and tested for extracellular ATP and cells were harvested and stained by flow cytometry for cell surface expression of calreticulin or HSP70. Data shown are the means of 3 independent experiments, with 3 replicates per experiment. <b>A,</b> Student’s t-test, <b>B-F</b>, one-way ANOVA. * p<0.05, ** p<0.01.