MIF expression in the primary tumor dampens anti-tumor T cell responses in vivo.

<p>1.0 x 10⁴ WT or MIF KD 4T1 cells were implanted in the mammary fat pad of female Balb/c mice. <b>A,</b> Tumors were harvested at day 10 of tumor growth and weighed. Tumors were then digested and dissociated, and cells were cultured <i>in vitro</i> for 4 hours in the presence of BFA +/- anti-CD3 stimulation. Cells were stained for CD4 and CD8 surface expression <b>(B)</b> and intracellular IFNgamma <b>(C)</b> by flow cytometry. Data shown are representative of one of three independent experiments, with n = 6 mice/group. One-way ANOVA. * p<0.05, ** p<0.01, ***p<0.001, **** p<0.0001.