EcoHIV establishes latent reservoirs in resting CD4⁺ cells that are inducible by epigenetic modulators.

<p><b>A.</b> CD4⁺ T cells isolated from spleen at day 25 post-infection were cocultured with uninfected cells in the presence or absence of ABC as indicated prior to vDNA amplification, each symbol represents culture from a single mouse. <b>B.</b> Six weeks after EcoHIV infection, CD4⁺ splenic T cells were harvested and purified from donor male 129X1/SvJ mice and injected into the recipient female 129X1 nude mice; their PM were collected after one week. Y chromosome and EcoHIV <i>gag</i> RNA levels in PM were determined by QPCR. Each symbol represents a single mouse. <b>C.-D.</b> Twelve weeks after infection, resting CD4⁺ T cells were purified from the spleen and <b>C.</b> The levels of integrated EcoHIV DNA and <b>D.</b> Genomic RNA were measured by QPCR. <b>E.</b> Eight weeks after EcoHIV infection of mice, resting CD4⁺ T cells were isolated and exposed to the agents indicated in culture for 2 days prior to collection and measurement of EcoHIV mRNA QPCR. <b>F.</b> Six weeks after infection by EcoHIV-Luc, mice were treated as shown, euthanized and splenic CD4⁺ cells isolated for measurement of virus RNA expression (left panel) or protein expression (right panel). *<i>P</i><0.05, **<i>P</i><0.01, ***<i>P</i><0.001.