Predicted mutational burden in individual stem cells with age.

<p><b>a)</b> We show simulated stochastic mutation accumulation in a stem cell population of constant size. Here <i>N</i> = 20,000 stem cells segregating DNA strands with probability <i>p</i> = 0.7 and a mutation rate of <i>μ</i> = 6 per cell division (corresponding to a mutation rate of <i>μ</i> = 10⁻⁹ per bp per cell division). <b>b)</b> Mutational burden and <b>c)</b> variance of the mutational burden increase linear. Linear regression (dashed lines) gives and . The expected exact values based on above parameters and Eqs (<a href="http://www.ploscompbiol.org/article/info:doi/10.1371/journal.pcbi.1006233#pcbi.1006233.e002" target="_blank">1</a>) and (<a href="http://www.ploscompbiol.org/article/info:doi/10.1371/journal.pcbi.1006233#pcbi.1006233.e003" target="_blank">2</a>) are and . Eqs (<a href="http://www.ploscompbiol.org/article/info:doi/10.1371/journal.pcbi.1006233#pcbi.1006233.e004" target="_blank">3</a>) and (<a href="http://www.ploscompbiol.org/article/info:doi/10.1371/journal.pcbi.1006233#pcbi.1006233.e005" target="_blank">4</a>) yield for the strand segregation probability <i>p</i> = 0.702 and for the mutation rate <i>μ</i> = 6.03, (exact values imposed on the simulation were <i>p</i> = 0.7 and <i>μ</i> = 6).