Additional file 6: of Identification of novel superoxide dismutase isoenzymes in the olive (Olea europaea L.) pollen

Figure S4. Construct maps SODc-pKB6 (5678 bp) and SODd-pKB6 (5654 bp) for protein expression of the two olive pollen sequences representative of the complete (accession no. EU250770.1) and deleted (EU250769.1) forms of a cytosolic Cu,Zn-SOD, respectively. Sequences were cloned in frame into the pKB6 vector (Rekom Biotech SL, Granada, Spain) using the BamHI y HindIII sites. The position of other restriction sites, and key signatures of the T7-P lac operon and kanamycin resistance genes are also displayed. (JPG 3265 kb