Wild type and mutant huntingtin protein expression in testis and striatum.

<p>Western blot analyses for huntingtin expression were performed in 3 months old BACHD rats and WT littermates. In order to validate the results, the blot was probed with anti HTT antibodies MAB2166 (mouse) and D7F7 (rabbit), recognizing distinct epitopes. Both antibodies detected both WT and mutant huntingtin. Ponceau red staining served to confirm equal loading of proteins. Note that WT HTT and mHTT expression levels were lower in testis than in striatum of BACHD rats. N = 3; fl-mhtt: full length mutant huntingtin; fl-wthtt: full length wild type huntingtin.