<p>(A) Representative images of 52 hpf <i>Tg(mpeg1</i>:<i>mCherry/mpx</i>:<i>EGFP)</i> embryos injected with antisense morpholino oligonucleotides to perturb the balance of neutrophil and macrophage populations. (B) <i>T</i>. <i>marneffei</i> CFU numbers at 24 hpi corresponding to the aligned treatment groups in panels (Ai-iv), for wild-type (WT) and myeloperoxidase-deficient (<i>mpx</i><sup>-/-</sup>) genotypes as shown. Data are mean±SEM for n≥3 experiments, n≥5 embryos/group/experiment.</p
<p>(Ai-iv) Histology of infected zebrafish embryos at different stages of <i>T</i>. <i>marneffei</i>...
Abstract Background Penicillium marneffei (P. marneffei) is a thermally dimorphic fungus pathogen th...
<p>(A) qRT-PCR measurement of <i>il8</i> transcripts during <i>Mabs</i> R- or S-infections (≈150 CFU...
<p>(A, B) Confocal imaging of leukocytes and calcofluor-stained acuD:RFP fungal conidia in <i>Tg(mpx...
<p>(A) Low magnification images of infected and uninfected whole <i>Tg(mpx</i>:<i>EGFP)</i> embryos ...
<p>(A) Ablation of macrophages decreases conidial viability in the first 24 hpi. (i) and (ii) show r...
<p><i>Tg(mpx</i>:<i>GFP)</i> embryos were injected at the one cell stage with either <i>csf3r</i> mo...
<p><b>a</b> Whole mount <i>in situ</i> hybridization of 2.5 dpf single and double <i>foxq1a</i><sup>...
<p><b>(A)</b> Embryo survival (left; representative experiment) and bacterial burden (total of 2 exp...
<p>(A-B-C-D) <i>Tg(mpx</i>:<i>Gal4/UAS</i>:<i>nfsB-mCherry/mpeg1</i>:<i>GFPcaax)</i> embryos were tr...
<p>(A) Double transgenic <i>Tg(mpeg</i>:<i>dendra2)</i> x <i>Tg(mpx</i>:<i>mCherry)</i> larvae show ...
<p>(A) Experimental scheme. One dpf <i>tg(mpeg1</i>:<i>mCherry-F/mpx</i>:<i>GFP) or tg(mfap4</i>:<i>...
<p>(A) <i>mfap4</i> RNA expression at 2 dpf shows a complete loss of macrophages in <i>irf8</i> muta...
(A) Tg(mpeg1:mCherry-F) embryos were injected intravenously at 30 h post fertilization with ~50 CFU ...
<p>(A) Schematic of the <i>Tol2-lyz</i>:<i>lta4h-2a-mCherry</i> construct that was injected into AB-...
<p>(Ai-iv) Histology of infected zebrafish embryos at different stages of <i>T</i>. <i>marneffei</i>...
Abstract Background Penicillium marneffei (P. marneffei) is a thermally dimorphic fungus pathogen th...
<p>(A) qRT-PCR measurement of <i>il8</i> transcripts during <i>Mabs</i> R- or S-infections (≈150 CFU...
<p>(A, B) Confocal imaging of leukocytes and calcofluor-stained acuD:RFP fungal conidia in <i>Tg(mpx...
<p>(A) Low magnification images of infected and uninfected whole <i>Tg(mpx</i>:<i>EGFP)</i> embryos ...
<p>(A) Ablation of macrophages decreases conidial viability in the first 24 hpi. (i) and (ii) show r...
<p><i>Tg(mpx</i>:<i>GFP)</i> embryos were injected at the one cell stage with either <i>csf3r</i> mo...
<p><b>a</b> Whole mount <i>in situ</i> hybridization of 2.5 dpf single and double <i>foxq1a</i><sup>...
<p><b>(A)</b> Embryo survival (left; representative experiment) and bacterial burden (total of 2 exp...
<p>(A-B-C-D) <i>Tg(mpx</i>:<i>Gal4/UAS</i>:<i>nfsB-mCherry/mpeg1</i>:<i>GFPcaax)</i> embryos were tr...
<p>(A) Double transgenic <i>Tg(mpeg</i>:<i>dendra2)</i> x <i>Tg(mpx</i>:<i>mCherry)</i> larvae show ...
<p>(A) Experimental scheme. One dpf <i>tg(mpeg1</i>:<i>mCherry-F/mpx</i>:<i>GFP) or tg(mfap4</i>:<i>...
<p>(A) <i>mfap4</i> RNA expression at 2 dpf shows a complete loss of macrophages in <i>irf8</i> muta...
(A) Tg(mpeg1:mCherry-F) embryos were injected intravenously at 30 h post fertilization with ~50 CFU ...
<p>(A) Schematic of the <i>Tol2-lyz</i>:<i>lta4h-2a-mCherry</i> construct that was injected into AB-...
<p>(Ai-iv) Histology of infected zebrafish embryos at different stages of <i>T</i>. <i>marneffei</i>...
Abstract Background Penicillium marneffei (P. marneffei) is a thermally dimorphic fungus pathogen th...
<p>(A) qRT-PCR measurement of <i>il8</i> transcripts during <i>Mabs</i> R- or S-infections (≈150 CFU...