Model validation using membrane potential data.

<p>Experiments were performed under the same experimental condition as in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0197921#pone.0197921.g004" target="_blank">Fig 4</a>. R123 buffer concentrations are shown (symbols). Either pyruvate + malate (PYR+MAL, blue, n = 4) or succinate (SUC, red, n = 4) was used as the metabolic substrate. Values are mean ± SE. Black lines in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0197921#pone.0197921.g008" target="_blank">Fig 8A</a> are model predictions using the estimated values of the model parameters in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0197921#pone.0197921.t002" target="_blank">Table 2</a>. <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0197921#pone.0197921.g008" target="_blank">Fig 8B</a> shows integrated model predictions of the mitochondrial membrane potential with either PYR + MAL or SUC as the metabolic substrates and with the values of the model parameters set to those in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0197921#pone.0197921.t002" target="_blank">Table 2</a>.