Model predicted substrate-dependent responses of the steady state reaction and transport fluxes.

<p>3-Dimensional surface plots of key metabolic fluxes at steady-state as a function of buffer pyruvate (PYR) and malate (MAL) concentrations ranging from 0.5 mM to 10 mM obtained at the end of a 10-minute incubation period in isolated lung mitochondria. Simulations were performed under the same experimental conditions as in Figs <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0197921#pone.0197921.g002" target="_blank">2</a> and <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0197921#pone.0197921.g003" target="_blank">3</a> with the values of the extrinsic model parameters set to those in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0197921#pone.0197921.t002" target="_blank">Table 2</a>.