Model validation using ACoA and CoA steady state data (Evans et al. [27]) and dynamic NADH redox status data [24].

<p><i>Panel A</i>. Rat lung mitochondria were incubated with fixed pyruvate (PYR) concentration (5 mM), and without or with 5 mM malate (MAL). Mitochondria density = 2.5 mg/ml. Other experimental conditions are the same as in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0197921#pone.0197921.g002" target="_blank">Fig 2</a>. ACoA and CoA steady-state concentrations were measured at the end of 10-min incubation time. Symbols are experimental data and solid lines are integrated model predictions under the same experimental conditions with the values of the model parameters set to those in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0197921#pone.0197921.t002" target="_blank">Table 2</a>. <i>Panel B</i>. Fluorescence of pyridine nucleotides (NADH) in isolated rat lung mitochondria. Mitochondrial density was 2.5 mg/ml. 2.5 mM PYR, 2.5 mM MAL and 0.2 mM ADP were added to the buffer at 0 min, 1 min and 2.2 min, respectively. Symbols are experimental data and solid lines are integrated model predictions under the same experimental conditions with the values of the model parameters set to those in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0197921#pone.0197921.t002" target="_blank">Table 2</a>.