Defects in NF-κB p50 nuclei accumulation by GSI treatment or deletion of CSL/RBP-Jκ in LPS/IC-activated macrophages.

<p>(A) BMMs were pretreated with DMSO or GSI for 1 and 4 hr and the cytosolic and nuclei fractions were analyzed for NF-κB p50 by Western blotting. GAPDH and CSL/RBP-Jκ were used as cytosolic and nuclei markers, respectively. (B) BMMs from wildtype or <i>Rbpj</i> KO mice were stimulated with LPS/IC for 1 and 4 hr and the cytosolic and nuclei fractions were analyzed for NF-κB p50 as described for (A). Representative data from 1 of 2 independent experiments are shown.