Representative picture for the determination of cytogenetic landmarks in the barley 5H chromatin.

<p><b>(A)</b> The hybridization signals of GISH and (<b>B)</b> FISH on the introgressed barley chromatin were visualized in the 5HS-7DS.7DL/Mv9Kr1/Mv Bodri genotype. (<b>C)</b> The hybridization signals of FISH on the intact 5H chromosome indicate the physical position of the HvT01, pTa71, (GAA)_n and (AGGGAG)_n repetitive probes in the barley genotype Igri. The positions of the fluorescence signals were expressed as fraction length (FL) on the barley chromatin from the centromere (0 FL) to the telomere (1 FL). The length of the 5HS chromosome arm corresponds to the distance between the telomere and the centromeric repeat (AGGGAG)_n. <b>(B, C)</b> The relative position of the satellite (pTa71 signal) is 0.692 FL, which is characterisitic of both intact and introgressed 5HS. <b>(C)</b> The region between the centromere-specific and pericentric (GAA)_n signals, which is missing from the 5HS-7DS.7DL translocation chromosome, represents the C-0.147 FL interval. <b>(A)</b> The relative position of the translocation breakpoint (TB) is 0.26 FL. The 1–0.26 FL region represents the transferred barley segment, accounting for 74% of the whole chromosome arm.