<p>(<b>A)</b> Cq values. The dashed line represents the average for all samples, which are described in more detail in Materials and Methods. Each condition was represented by two biological replicates, each analyzed with three technical replicates. Error bars represent standard deviations. (<b>B)</b> ranking of performance of each primer set based on the Normfinder, geNorm, delta Ct, and BestKeeper algorithms, plus a comprehensive ranking based on the RefFinder program.</p
Sequences of primers used for RT-qPCR to assess the expression of the indicated genes.</p
<p>*: PCR efficiency (calculated from the standard curve)</p><p>**: Regression coefficient</p><p>†: ...
<p><b>A.</b> All samples (all tissues, all developmental stages). <b>B.</b> Pulp samples (all develo...
<p>Genes ranked by stability based on (<b>A</b>) CV, (<b>B</b>) geNorm and (<b>C</b>) NormFinder too...
The box includes all data points between the 25% quantile and the 75% quantile. The whiskers span al...
<p>The primers used for RT-qPCR analysis of the genes assessed in this study, including the gene sym...
<p>* Commonly used reference genes.</p><p>RT-qPCR primer sets used in this study and their target ge...
<p>(<b>A</b>). Line chart of all publications (n = 37) investigating the most stably expressed refer...
<p>Ct values for each reference gene in all Hyperaccumulating ecotype of <i>S. alfredii</i> samples....
a<p>: F, forward primer; R, reverse primer.</p>b<p>: Tm, Melting temperature.</p>c<p>: E, Efficiency...
<p>RT-qPCR confirmation (left Y-axis, green bars) and normalized RNA-seq read count values (right Y-...
<p>“*”: PCR efficiency (calculated from the standard curve).</p><p>“**”: Regression coefficient.</p>...
<p>A boxplot of RNA-Seq data showing the expression levels of 37 previously reported primer sets fro...
<p>Candidate reference genes, primers and different parameters derived from RT-qPCR analysis.</p
<p>Stability analysis in (A) Liver, (B) Breast, (C) Colon and (D) All cancer cell lines. NormFinder ...
Sequences of primers used for RT-qPCR to assess the expression of the indicated genes.</p
<p>*: PCR efficiency (calculated from the standard curve)</p><p>**: Regression coefficient</p><p>†: ...
<p><b>A.</b> All samples (all tissues, all developmental stages). <b>B.</b> Pulp samples (all develo...
<p>Genes ranked by stability based on (<b>A</b>) CV, (<b>B</b>) geNorm and (<b>C</b>) NormFinder too...
The box includes all data points between the 25% quantile and the 75% quantile. The whiskers span al...
<p>The primers used for RT-qPCR analysis of the genes assessed in this study, including the gene sym...
<p>* Commonly used reference genes.</p><p>RT-qPCR primer sets used in this study and their target ge...
<p>(<b>A</b>). Line chart of all publications (n = 37) investigating the most stably expressed refer...
<p>Ct values for each reference gene in all Hyperaccumulating ecotype of <i>S. alfredii</i> samples....
a<p>: F, forward primer; R, reverse primer.</p>b<p>: Tm, Melting temperature.</p>c<p>: E, Efficiency...
<p>RT-qPCR confirmation (left Y-axis, green bars) and normalized RNA-seq read count values (right Y-...
<p>“*”: PCR efficiency (calculated from the standard curve).</p><p>“**”: Regression coefficient.</p>...
<p>A boxplot of RNA-Seq data showing the expression levels of 37 previously reported primer sets fro...
<p>Candidate reference genes, primers and different parameters derived from RT-qPCR analysis.</p
<p>Stability analysis in (A) Liver, (B) Breast, (C) Colon and (D) All cancer cell lines. NormFinder ...
Sequences of primers used for RT-qPCR to assess the expression of the indicated genes.</p
<p>*: PCR efficiency (calculated from the standard curve)</p><p>**: Regression coefficient</p><p>†: ...
<p><b>A.</b> All samples (all tissues, all developmental stages). <b>B.</b> Pulp samples (all develo...