Variation in SNP sequences: Contrast between consistent germline (buccal or blood) cells and the variation in sequences from 116 skin surface scrapings from different anatomic sites in 12 normal donors.

<p>DNA from each donor was extracted from separate buccal swabs (left or right sides) and blood and analyzed for presence of <i>SLC24A5</i> rs1426654 (A/G) allele. Paired buccal and/or blood samples from the same donor had the same germline genotypes. Multiple skin surface scrapings at different anatomic sites were obtained from normal donors of different ages, genders, and ethnicities. Different SNP sequences were identified at different sites in the same donor. DNA alleles from all samples were determined by Sanger sequencing (see details in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0198011#sec014" target="_blank">Methods</a>).